Robust expertise in the field of immunoassays, molecular and cell biology and biochemistry attending upon discovery and development of new products. Long-standing skills on development and application of method for detection of large molecules in complex matrices, MoA investigation, target validation.
Techniques
Cell based assays (e.g., viability, cytotoxicity, apoptosis, permeability, chemotaxis, mineralization, reporter gene assay)
Genotoxicity assay (AMES test)
Immunohistochemistry, immunofluorescence and immunocytochemistry
Recombinant DNA techniques
Lab-scale recombinant protein production, purification and testing
PCR
Real Time qPCR
ELISA
SDS-PAGE
1DE, 2DE Western Blotting
Dot Blot
Equipment
Multiplate readers: M1000PRO, TECAN and Sunrise, TECAN
Real Time PCR: LC480, Roche and 7500, Applied Biosystems
Spectrophotometer: Nanodrop ND1000, Euroclone
Cell Incubators: HERAcell, Thermo and BB16, Heraeus
Laminar Flow Hoods: BSB-4, Labosystems and Aura2000MAC, Bioair
RNA Extractor: MagMAX Express, Applied Biosystems
Confocal microscope: TCS SPE scan head RGB, Leica
FPLC system GEHealthcare/Amersham ÄKTA Explorer with UV/visible, multi-wavelenght and conductivity detectors
Molecular Imager: Versa Doc 4000MP, BioRad
