In vitro Target Validation – Signal pathway investigation
Stable or transient cell transfection
Biomarker discovery and detection
Gene expression analysis
Quantitative detection method set up (immunoenzymatic, mass spectrometry, real time PCR)
Protein identification and characterization
MALDI imaging of endogenous or exogenous unlabeled compounds (small molecules and proteins) (case study).
Peptide mass fingerprinting by protease digestion (proteins are digested with trypsin and the resulting peptides are analyzed by LC-MS/MS).
Protein mapping (processing with multiple enzymes, identification reported for individual enzymes).
Proteome and phospho-proteome qualitative and semi-quantitative analysis by 2-DE followed by image analysis.
Phosphorylation and glycosylation sites determination through MS/MS (from a high purity sample).
Protein/proteome profiling (qualitative profile of a complex sample).
ImmunoPrecipitation-profiling (ID of immunoprecipitated or co-immunoprecipitated proteins; these experiments can be used to profile the protein content of a sample).